hdmecs 2000 Search Results


hdmecs 2000  (ScienCell)
90
ScienCell hdmecs 2000
High levels of barrier resistance correlate with continuous intercellular junctions and cortical arrangement of the actin cytoskeleton. (A) Confocal images of confluent endothelial monolayers displayed in order of low (HAEC, HBMVEC, and HUVEC) and high levels of barrier resistance (HLMVEC and HDMEC) left to right (bar, 20 µm; representative of n = 3). HLMVEC and HDMEC exhibit a linear and organized junctional staining of VE-cadherin, claudin-5, and ZO-1 compared with an irregular junctional pattern in cell types with lower levels of resistance. Phalloidin staining reveals a strict cortical arrangement of the actin cytoskeleton in HLMVEC and HDMEC, whereas cells with low resistance exhibit more stress fibers. Localization of cortactin is more peripheral and less cytoplasmic <t>in</t> <t>HLMVECs</t> and <t>HDMECs.</t> F-actin fibers aligned along the cell periphery, minimizing radial tension forces at cell junctions, indicated by linear junctional pattern of VE-cadherin/phalloidin overlay images (arrows) and lower presence of pMLC2 in HLMVECs and HDMECs. ( B–F) Graphs presenting mean values of fluorescence intensity across multiple cell–cell junctions per cell type (Fig. S2 B) for each barrier protein shown in A. Junctional components as well as phalloidin and cortactin are concentrated along the cell borders in HLMVECs and HDMECs compared with cell types with low barrier resistance.
Hdmecs 2000, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hdmecs+2000/pmc06080943-227-44-48?v=ScienCell
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hdmecs 2000 - by Bioz Stars, 2026-08
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hdmec cell line 2000  (ScienCell)
90
ScienCell hdmec cell line 2000
High levels of barrier resistance correlate with continuous intercellular junctions and cortical arrangement of the actin cytoskeleton. (A) Confocal images of confluent endothelial monolayers displayed in order of low (HAEC, HBMVEC, and HUVEC) and high levels of barrier resistance (HLMVEC and HDMEC) left to right (bar, 20 µm; representative of n = 3). HLMVEC and HDMEC exhibit a linear and organized junctional staining of VE-cadherin, claudin-5, and ZO-1 compared with an irregular junctional pattern in cell types with lower levels of resistance. Phalloidin staining reveals a strict cortical arrangement of the actin cytoskeleton in HLMVEC and HDMEC, whereas cells with low resistance exhibit more stress fibers. Localization of cortactin is more peripheral and less cytoplasmic <t>in</t> <t>HLMVECs</t> and <t>HDMECs.</t> F-actin fibers aligned along the cell periphery, minimizing radial tension forces at cell junctions, indicated by linear junctional pattern of VE-cadherin/phalloidin overlay images (arrows) and lower presence of pMLC2 in HLMVECs and HDMECs. ( B–F) Graphs presenting mean values of fluorescence intensity across multiple cell–cell junctions per cell type (Fig. S2 B) for each barrier protein shown in A. Junctional components as well as phalloidin and cortactin are concentrated along the cell borders in HLMVECs and HDMECs compared with cell types with low barrier resistance.
Hdmec Cell Line 2000, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hdmecs+2000/pm20545324-46-8-14?v=ScienCell
Average 90 stars, based on 1 article reviews
hdmec cell line 2000 - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


High levels of barrier resistance correlate with continuous intercellular junctions and cortical arrangement of the actin cytoskeleton. (A) Confocal images of confluent endothelial monolayers displayed in order of low (HAEC, HBMVEC, and HUVEC) and high levels of barrier resistance (HLMVEC and HDMEC) left to right (bar, 20 µm; representative of n = 3). HLMVEC and HDMEC exhibit a linear and organized junctional staining of VE-cadherin, claudin-5, and ZO-1 compared with an irregular junctional pattern in cell types with lower levels of resistance. Phalloidin staining reveals a strict cortical arrangement of the actin cytoskeleton in HLMVEC and HDMEC, whereas cells with low resistance exhibit more stress fibers. Localization of cortactin is more peripheral and less cytoplasmic in HLMVECs and HDMECs. F-actin fibers aligned along the cell periphery, minimizing radial tension forces at cell junctions, indicated by linear junctional pattern of VE-cadherin/phalloidin overlay images (arrows) and lower presence of pMLC2 in HLMVECs and HDMECs. ( B–F) Graphs presenting mean values of fluorescence intensity across multiple cell–cell junctions per cell type (Fig. S2 B) for each barrier protein shown in A. Junctional components as well as phalloidin and cortactin are concentrated along the cell borders in HLMVECs and HDMECs compared with cell types with low barrier resistance.

Journal: The Journal of Cell Biology

Article Title: Vav3-induced cytoskeletal dynamics contribute to heterotypic properties of endothelial barriers

doi: 10.1083/jcb.201706041

Figure Lengend Snippet: High levels of barrier resistance correlate with continuous intercellular junctions and cortical arrangement of the actin cytoskeleton. (A) Confocal images of confluent endothelial monolayers displayed in order of low (HAEC, HBMVEC, and HUVEC) and high levels of barrier resistance (HLMVEC and HDMEC) left to right (bar, 20 µm; representative of n = 3). HLMVEC and HDMEC exhibit a linear and organized junctional staining of VE-cadherin, claudin-5, and ZO-1 compared with an irregular junctional pattern in cell types with lower levels of resistance. Phalloidin staining reveals a strict cortical arrangement of the actin cytoskeleton in HLMVEC and HDMEC, whereas cells with low resistance exhibit more stress fibers. Localization of cortactin is more peripheral and less cytoplasmic in HLMVECs and HDMECs. F-actin fibers aligned along the cell periphery, minimizing radial tension forces at cell junctions, indicated by linear junctional pattern of VE-cadherin/phalloidin overlay images (arrows) and lower presence of pMLC2 in HLMVECs and HDMECs. ( B–F) Graphs presenting mean values of fluorescence intensity across multiple cell–cell junctions per cell type (Fig. S2 B) for each barrier protein shown in A. Junctional components as well as phalloidin and cortactin are concentrated along the cell borders in HLMVECs and HDMECs compared with cell types with low barrier resistance.

Article Snippet: Specifically, we obtained HUVECs (C2519A; Lonza; C-12203; PromoCell), HSaVECs (HSVEC/A; VEC-Technologies; cAP-0019; Angio-Proteomie), HAECs (PCS-100-011; ATCC; 6100; ScienCell), HIAECs (CC-2545; Lonza; cAP-0020; Angio-Proteomie), HBMVECs (ACBRI 376 V; Cell-Systems; cAP-0002; Angio-Proteomie), HUMVECs (C-12295; PromoCell; 7000; ScienCell), HLMVECs (3000; ScienCell; C-12281; PromoCell), HAMVECs (7200; ScienCell), and HDMECs (C-12212; PromoCell; 2000; ScienCell).

Techniques: Staining, Fluorescence

Knockdown of VAV3 reduces barrier strength and alters cytoskeletal arrangement in HDMECs and HLMVECs. (A) siRNA knockdown of three candidate genes with a strong correlation of expression to barrier resistance ( BAIAP2, VAV3, and SORBS2 ) validates the effect of VAV3 as an important regulator. (B) Efficiency of siRNA knockdown for BAIAP2, VAV3, and SORBS2 . (C and E) Effect of VAV3 silencing on barrier resistance compared with combined siRNA knockdown of VAV2 and VAV3 or CLDN5 (positive control) in HDMECs and HLMVECs. (D and F) Efficiency of siRNA knockdown for VAV2 , VAV3 , and CLDN5 (ECIS dataset C and E) in HDMECs and HLMVECs versus control siRNA. (G and H) Bar graphs of barrier resistance levels at 48 h for HDMECs and HLMVECs (ECIS dataset C and E). Error bars show mean ± SEM; *, P < 0.05; n = 3. (I) Table presenting ECIS data modeling values of R b , α , and C m at 48 h for HDMECs and HLMVECs with siRNA knockdown for VAV3 , VAV2/3 , and CLDN5 versus control siRNA, respectively (mean ± SEM; n = 3). (J) Immunofluorescence staining of VE-cadherin, ZO-1, F-actin (phalloidin), and cortactin in HDMEC monolayers subjected to either siRNA knockdown of VAV3 and VAV2/3 or siRNA control (bars, 20 µm). Magnification of phalloidin staining highlights loss of cortical actin and gain of stress fibers upon VAV3 and VAV2/3 knockdown. Translocation of cortactin to the cell periphery (filled arrows) is reduced in monolayers with siRNA knockdown of either VAV3 alone or VAV2/3 (open arrows). (K) Fluorescence intensity across cell–cell junctions (mean of n = 6) in cells exposed to siRNA control, siRNA VAV3 , and siRNA VAV2/3 (for VE-cadherin, ZO-1, phalloidin, and cortactin; as shown in J).

Journal: The Journal of Cell Biology

Article Title: Vav3-induced cytoskeletal dynamics contribute to heterotypic properties of endothelial barriers

doi: 10.1083/jcb.201706041

Figure Lengend Snippet: Knockdown of VAV3 reduces barrier strength and alters cytoskeletal arrangement in HDMECs and HLMVECs. (A) siRNA knockdown of three candidate genes with a strong correlation of expression to barrier resistance ( BAIAP2, VAV3, and SORBS2 ) validates the effect of VAV3 as an important regulator. (B) Efficiency of siRNA knockdown for BAIAP2, VAV3, and SORBS2 . (C and E) Effect of VAV3 silencing on barrier resistance compared with combined siRNA knockdown of VAV2 and VAV3 or CLDN5 (positive control) in HDMECs and HLMVECs. (D and F) Efficiency of siRNA knockdown for VAV2 , VAV3 , and CLDN5 (ECIS dataset C and E) in HDMECs and HLMVECs versus control siRNA. (G and H) Bar graphs of barrier resistance levels at 48 h for HDMECs and HLMVECs (ECIS dataset C and E). Error bars show mean ± SEM; *, P < 0.05; n = 3. (I) Table presenting ECIS data modeling values of R b , α , and C m at 48 h for HDMECs and HLMVECs with siRNA knockdown for VAV3 , VAV2/3 , and CLDN5 versus control siRNA, respectively (mean ± SEM; n = 3). (J) Immunofluorescence staining of VE-cadherin, ZO-1, F-actin (phalloidin), and cortactin in HDMEC monolayers subjected to either siRNA knockdown of VAV3 and VAV2/3 or siRNA control (bars, 20 µm). Magnification of phalloidin staining highlights loss of cortical actin and gain of stress fibers upon VAV3 and VAV2/3 knockdown. Translocation of cortactin to the cell periphery (filled arrows) is reduced in monolayers with siRNA knockdown of either VAV3 alone or VAV2/3 (open arrows). (K) Fluorescence intensity across cell–cell junctions (mean of n = 6) in cells exposed to siRNA control, siRNA VAV3 , and siRNA VAV2/3 (for VE-cadherin, ZO-1, phalloidin, and cortactin; as shown in J).

Article Snippet: Specifically, we obtained HUVECs (C2519A; Lonza; C-12203; PromoCell), HSaVECs (HSVEC/A; VEC-Technologies; cAP-0019; Angio-Proteomie), HAECs (PCS-100-011; ATCC; 6100; ScienCell), HIAECs (CC-2545; Lonza; cAP-0020; Angio-Proteomie), HBMVECs (ACBRI 376 V; Cell-Systems; cAP-0002; Angio-Proteomie), HUMVECs (C-12295; PromoCell; 7000; ScienCell), HLMVECs (3000; ScienCell; C-12281; PromoCell), HAMVECs (7200; ScienCell), and HDMECs (C-12212; PromoCell; 2000; ScienCell).

Techniques: Knockdown, Expressing, Positive Control, Control, Immunofluorescence, Staining, Translocation Assay, Fluorescence